PCR-based Specific Detection of Bacillus in Liquid Organic Fertilizer
Penulis:
Rapid molecular PCR-based detection method for Bacillus species used in the production of Beyonic Accepted May 31, 2019/ Published online June 24, 2019 *Corresponding Author: Iman Hidayat – e-mail – imanhidayat@yahoo.com Corresponding Author: Iman Hidayat – e-mail – imanhidayat@yahoo.com ® liquid organic fertilizer was carried out based on nucleotide sequence data from the 16S rRNA gene. The method involved sequencing the 16S rRNA gene of several Bacillus species and identifying around 16-22 specific nucleotide bases from 5' and 3' ends in the Bacillus 16S rRNA gene sequences. One specific primer pair for Bacillus detection was determined as follow: 5' - CAT AAG ACT GGG ATA ACT CCG GG - 3' (forward) from positions of 85-107 bp, and 5’ - CCA GGC GGA GTG CTT AAT GC - 3’ (reverse) from positions of 836-854 bp. PCR assay and gel electrophoresis analysis showed that the primer pair was specific to the genus Bacillus. Keywords: bacteria, detection, molecular marker, organic fertilizer, 16S rRNAJournal of Microbial Systematics and Biotechnology 1(1), 44-47
ISSN / ISBN / IBSN : 2685-4430
No. Arsip : LIPI-20191029
Download Disini