Alkyl β-D-xyloside synthesis from black liquor xylan using Aureobasidium pullulans CBS 135684 β-xylosidases immobilized on spent expanded perlite

Penulis: Dede Heri Yuli Yanto,Wichanee Bankeeree, Takashi Watanabe, Hunsa Punnapayak, Pongtharin Lotrakul, Sehanat Prasongsuk, Ruibo Li.

The crude β-xylosidase from Aureobasidium pullulans CBS 135684 was immobilized on the surface of the modified spent expanded perlite via (3-aminopropyl) triethoxy-silane and glutaraldehyde cross-linking reaction. Over 80% of the initial free enzyme activity was detected on the bound enzyme. The immobilized β-xylosidase (204.7 ± 6.5 U g-1 of perlite) exhibited transxylosylation activity when sugars from black liquor xylan hydrolysate and a variety of alcohols (C1-6) were used as donor and acceptor molecules, respectively, yielding a number of alkyl xylosides. The synthesized hexyl xyloside exhibited some interesting properties comparable with those of a commercial hexyl glucoside, including an antioxidant activity (IC50 = 8.9 ± 2.0 mg mL-1), wetting time (43 s), and critical micelle concentration value (252 mmol L-1). Optimization for hexyl xyloside synthesis was performed via statistical analysis using a Box and Behnken design. The maximum predicted yield of hexyl xyloside (499.0 ± 1.1 mg g-1 xylan hydrolysate) was obtained with 10% (w/v) xylan hydrolysate, 10 U g-1 immobilized β-xylosidase, and 13% (v/v) hexanol at 70 °C, pH 6.0 for 4 h, and it was validated by the following experimental yield (498.9 ± 4.8 mg g-1 xylan hydrolysate). The maximum production yield remained rela- tively stable after the immobilized enzyme was reused for 12 cycles with a 471.5 ± 15.1 mg g-1 hexyl xyloside yield (94.4% of the first cycle). Thus, the immobilized β-xylosidase has a high potential as a robust catalyst for application in alkyl xyloside production.

W. Bankeeree, T. Watanabe, H. Punnapayak, P. Lotrakul, S. Prasongsuk, R. Li, D.H.Y. Yanto. 2020. https://doi.org/10.1007/s13399-020-00755-5 Alkyl β-D-xyloside synthesis from black liquor xylan using Aureobasidium pullulans CBS 135684 β-xylosidases immobilized on spent expanded perlite. Biomass Conversion and Biorefinery.

https://link.springer.com/article/10.1007/s13399-020-00755-5

ISSN / ISBN / IBSN : 2190-6823

DOI: https://doi.org/10.1007/s13399-020-00755-5

No. Arsip : LIPI-20200530